Effects of H-7 on the iris and ciliary muscle in monkeys

Arch Ophthalmol. 1998 Aug;116(8):1070-7. doi: 10.1001/archopht.116.8.1070.


Objectives: To determine the effects of H-7 on (1) iris and ciliary muscles (CMs) in living monkeys; (2) isolated monkey CM strips; (3) actomyosin contractility in cultured Swiss 3T3 cells.

Methods: (1) Pupillary diameter (calipers) and accommodation (refractometer) in living monkeys were measured after topical, intracameral, or intravitreal administration of H-7 followed by systemic pilocarpine hydrochloride. (2) Pilocarpine-induced contraction of isolated monkey CM strips following administration of H-7 was measured in a perfusion chamber. (3) Actomyosin contractility in Swiss 3T3 cells cultured on thin silicone rubber film was determined by measuring cell-induced film wrinkles before and after administration of H-7.

Results: Topical H-7 prevented anesthesia-induced miosis but did not affect resting refraction. Intracameral or intravitreal H-7 dilated the pupil and inhibited miotic but not accommodative responses to pilocarpine. H-7 inhibited pilocarpine-induced contraction of isolated monkey CM strips and reduced Swiss 3T3 cell contraction.

Conclusions: H-7 inhibits actin-based contractility in non-muscle cells and in monkey iris sphincter and CM. Under our in vivo experimental conditions, the effect on the iris predominates over that on the CM.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology*
  • 3T3 Cells / drug effects
  • 3T3 Cells / metabolism
  • Accommodation, Ocular / drug effects
  • Actomyosin / metabolism
  • Animals
  • Cells, Cultured
  • Ciliary Body / drug effects*
  • Enzyme Inhibitors / pharmacology*
  • Humans
  • Iris / drug effects*
  • Macaca fascicularis
  • Mice
  • Muscle Contraction / drug effects
  • Muscle, Smooth / drug effects*
  • Ophthalmic Solutions
  • Pilocarpine / pharmacology
  • Protein Kinase Inhibitors
  • Pupil / drug effects
  • Refraction, Ocular / drug effects


  • Enzyme Inhibitors
  • Ophthalmic Solutions
  • Protein Kinase Inhibitors
  • Pilocarpine
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Actomyosin