Sex-associated differences in cold-induced UCP1 synthesis in rodent brown adipose tissue

Pflugers Arch. 1998 Oct;436(5):689-95. doi: 10.1007/s004240050690.

Abstract

The effects of acute and chronic acclimation to cold on uncoupling protein 1 (UCP1) levels, as well as on GDP-binding to mitochondria, cytochrome c oxidase activity and mitochondrial protein concentration in brown adipose tissue (BAT) of intact male and female rats have been analyzed. Results reveal that females rats are more sensitive to cold because their threshold temperature for the thermogenic response is set at a higher value (around 22 degreesC) than that of males (around 18 degreesC), hence leading to differences in BAT UCP1 levels between the sexes at different environmental temperatures. In vitro experiments showed that steroid hormones, beta-estradiol, estrone and progesterone, can reduce norepinephrine-induced UCP1 synthesis in brown adipocytes differentiated in primary culture. Thus the different sex-associated response of cold-induced thermogenesis in rats does not appear to be explained by a direct action of sex steroids upon the adipocyte, implying that other factors in the thermogenic regulatory system must be involved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / cytology
  • Adipose Tissue, Brown / metabolism*
  • Adipose Tissue, Brown / physiology
  • Animals
  • Body Temperature Regulation / physiology
  • Body Weight / physiology
  • Carrier Proteins / analysis
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / drug effects
  • Cells, Cultured
  • Cold Temperature*
  • Environmental Exposure
  • Estradiol / pharmacology
  • Estrone / pharmacology
  • Female
  • Guanosine Diphosphate / metabolism
  • Ion Channels
  • Male
  • Membrane Proteins / analysis
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / drug effects
  • Mice
  • Mitochondria / metabolism
  • Mitochondrial Proteins
  • Norepinephrine / pharmacology
  • Progesterone / pharmacology
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / drug effects
  • Rats
  • Sex Factors
  • Temperature
  • Uncoupling Agents
  • Uncoupling Protein 1

Substances

  • Carrier Proteins
  • Ion Channels
  • Membrane Proteins
  • Mitochondrial Proteins
  • RNA, Messenger
  • Ucp1 protein, mouse
  • Ucp1 protein, rat
  • Uncoupling Agents
  • Uncoupling Protein 1
  • Guanosine Diphosphate
  • Estrone
  • Progesterone
  • Estradiol
  • Norepinephrine