Electrophoretic analyses in a case of monoclonal gamma chain disease

Electrophoresis. 1998 Jul;19(10):1771-3. doi: 10.1002/elps.1150191038.

Abstract

Abnormal low molecular weight immunoglobulin heavy chain can be detected in serum samples from patients with heavy chain disease. In this paper, we report the characterization of a gamma heavy chain in a patient suffering from a lymphoplasmocytic disorder, using immunofixation analysis and two-dimensional polyacrylamide gel electrophoresis. We demonstrate that the combination of serum protein agarose electrophoresis and two-dimensional electrophoresis (three-dimensional electrophoresis) can be used to further characterize abnormal protein bands detected by immunofixation.

Publication types

  • Case Reports

MeSH terms

  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Female
  • Heavy Chain Disease / diagnosis
  • Heavy Chain Disease / immunology*
  • Humans
  • Immunoglobulin gamma-Chains / analysis*
  • Lymphatic Diseases / immunology
  • Lymphatic Diseases / surgery
  • Male
  • Middle Aged

Substances

  • Immunoglobulin gamma-Chains