The Lipopolysaccharide Biosynthesis Locus of Campylobacter Jejuni 81116

Microbiology. 1998 Aug;144 ( Pt 8):2049-61. doi: 10.1099/00221287-144-8-2049.

Abstract

Most Campylobacter jejuni strains express lipo-oligosaccharides. Some strains also express lipopolysaccharides (LPS), with O-antigen-like carbohydrate repeats. C. jejuni 81116 expresses an LPS containing both lipo-oligosaccharides and O-antigen-like repeats, but nothing is known about the structure or sugar composition of these LPS species. A cosmid library of the genome of C. jejuni 81116 was constructed and probed with Campylobacter hyoilei genes involved in LPS synthesis. Five cosmids hybridized with the probe and two of these expressed C. jejuni 81116 LPS in Escherichia coli. By subcloning, a 16 kb DNA region was identified which contains the genetic information required to express C. jejuni LPS. DNA sequence analysis revealed 11 ORFs homologous to genes involved in LPS synthesis of other bacteria. They consisted of three homologues of sugar biosynthesis genes, two homologues of transport genes and six homologues of sugar transferases.

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • Acetyltransferases / genetics
  • Amino Acid Sequence
  • Bacterial Proteins*
  • Campylobacter jejuni / genetics*
  • Campylobacter jejuni / metabolism*
  • Carbohydrate Epimerases / genetics
  • DNA, Bacterial / isolation & purification
  • Escherichia coli Proteins*
  • Galactosyltransferases / genetics
  • Genes, Bacterial*
  • Glycosyltransferases / genetics
  • Lipopolysaccharides / biosynthesis*
  • Lipopolysaccharides / chemistry
  • Membrane Proteins / genetics
  • Methyl-Accepting Chemotaxis Proteins
  • Molecular Sequence Data
  • Multigene Family
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transaminases / genetics
  • Transferases (Other Substituted Phosphate Groups) / genetics
  • UDPglucose 4-Epimerase / genetics

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • DNA, Bacterial
  • Escherichia coli Proteins
  • Lipopolysaccharides
  • Membrane Proteins
  • Methyl-Accepting Chemotaxis Proteins
  • wlaB protein, Campylobacter jejuni
  • Acetyltransferases
  • NeuD protein, E coli
  • Glycosyltransferases
  • Galactosyltransferases
  • wlaG protein, Campylobacter jejuni
  • lipooligosaccharide 1,5-heptosyltransferase
  • Transaminases
  • Transferases (Other Substituted Phosphate Groups)
  • undecaprenyl-phosphate galactosephosphotransferase
  • Carbohydrate Epimerases
  • perosamine synthetase
  • UDPglucose 4-Epimerase
  • galactose epimerase

Associated data

  • GENBANK/Y11648