The structure of the exocellular polysaccharide produced by the Archaeon Haloferax gibbonsii (ATCC 33959)

Carbohydr Res. 1998 Jun;309(1):89-94. doi: 10.1016/s0008-6215(98)00102-5.

Abstract

The structure of the neutral exocellular polysaccharide isolated from the Archaeon Haloferax gibbonsii (ATCC 33959) has been determined using acid hydrolysis, methylation analysis and NMR spectroscopy. The polysaccharide contained D-Man, D-Glc, D-Gal and L-Rha in the ratios 2:1:3:1. The substitution patterns of the sugar residues were deduced from the methylation analysis which indicated the polymer to be composed of a heptasaccharide repeating unit containing two branches. The 1H and 13C NMR resonances of the component sugars were assigned using COSY, HOHAHA, HMQC, and HMQC-TOCSY 2D NMR experiments and the sequence of the sugars in the repeating unit was determined from NOESY and HMBC experiments. The structure can be written as: [formula: see text]

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Carbohydrates / analysis
  • Haloferax / chemistry*
  • Hydrolysis
  • Methylation
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • Oligosaccharides / chemistry*
  • Oligosaccharides / isolation & purification
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification

Substances

  • Carbohydrates
  • Oligosaccharides
  • Polysaccharides