The impact of two-hybrid and related methods on biotechnology

Trends Biotechnol. 1998 Aug;16(8):355-63. doi: 10.1016/s0167-7799(98)01225-6.


Two-hybrid technology has contributed significantly to the unraveling of molecular regulatory networks by facilitating the discovery of protein interactions. Outgrowths of these methods are developing rapidly, including interaction mating to identify false positives and map protein networks, two-bait systems, systems not based on transcription, and systems permitting the selection of peptide aptamers to manipulate gene and allele function. These advances promise to have a significant impact on industrial biotechnology and drug development.

Publication types

  • Review

MeSH terms

  • Biotechnology / trends*
  • DNA-Binding Proteins / genetics*
  • Genes, Reporter*
  • Genetic Engineering / trends*
  • Humans
  • Protein Binding


  • DNA-Binding Proteins