Nicotine stereoisomers and cotinine stimulate prostaglandin E2 but inhibit thromboxane B2 and leukotriene E4 synthesis in whole blood

Eur J Pharmacol. 1998 Jul 17;353(1):87-92. doi: 10.1016/s0014-2999(98)00384-7.


The effects of (-)-nicotine (0.0005-500 microM), (+)-nicotine (0.0005-50 microM) and (-)-cotinine (0.0005-500 microM) on arachidonic acid metabolism were investigated in Ca2+ ionophore A23187 (calcimycin)-stimulated human whole blood in vitro. (-)-Nicotine and (-)-cotinine stimulated prostaglandin E2 but inhibited thromboxane B2 synthesis, as has been observed previously in A23187-stimulated polymorphonuclear leukocytes and platelet-rich plasma [Saareks, V., Riutta, A., Mucha, I., Alanko, J., Vapaatalo, H., 1993. Nicotine and cotinine modulate eicosanoid production in human leukocytes and platelet rich plasma. Eur. J. Pharmacol., 248, 345-349.]. (+)-Nicotine also stimulated prostaglandin E2 but inhibited thromboxane B2 synthesis. High concentrations of (-)-nicotine and (-)-cotinine and even nanomolar concentrations of (+)-nicotine inhibited leukotriene E4 synthesis. These results indicate that (-)-nicotine and (-)-cotinine stimulate cyclooxygenase but inhibit thromboxane synthase and 5-lipoxygenase in whole blood in vitro. (+)-Nicotine is capable of affecting in the same direction as well.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Arachidonate 5-Lipoxygenase / blood
  • Arachidonic Acid / metabolism
  • Calcimycin / pharmacology
  • Cotinine / pharmacology*
  • Dinoprostone / biosynthesis*
  • Dinoprostone / blood
  • Humans
  • In Vitro Techniques
  • Ionophores / pharmacology
  • Leukotriene E4 / biosynthesis*
  • Leukotriene E4 / blood
  • Middle Aged
  • Nicotine / chemistry
  • Nicotine / pharmacology*
  • Prostaglandin-Endoperoxide Synthases / blood
  • Stereoisomerism
  • Thromboxane B2 / biosynthesis*
  • Thromboxane B2 / blood


  • Ionophores
  • Arachidonic Acid
  • Calcimycin
  • Thromboxane B2
  • Nicotine
  • Leukotriene E4
  • Arachidonate 5-Lipoxygenase
  • Prostaglandin-Endoperoxide Synthases
  • Cotinine
  • Dinoprostone