Intradermal leukotriene B4, but not prostaglandin E2, induces itch-associated responses in mice

Eur J Pharmacol. 1998 Jul 17;353(1):93-6. doi: 10.1016/s0014-2999(98)00440-3.


The itch-associated responses induced by intradermal injection of leukotriene B4 and prostaglandin E2 were studied in mice. Leukotriene B4(0.001-1 nmol/site) elicited scratching of the injected site; the dose-response curve was bell-shaped with a peak effect at 0.03 nmol/site. The effect of leukotriene B4 (0.03 nmol/site) started within 3 min, peaked in the second 10-min period, had almost subsided by 30 min, and was inhibited by the simultaneous injection of the leukotriene B4 receptor antagonist ONO-4057, 5-[2-(2carboxyethyl)-3-(6-( p-methoxyphenyl)-5E-hexenyl) oxyphenyoxy] valeric acid. Prostaglandin E2 (0.003-300 nmol/site) did not significantly elicit scratching. The results raise the possibility that leukotriene B4 is an endogenous itch mediator in the skin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dinoprostone / administration & dosage
  • Dinoprostone / metabolism*
  • Dinoprostone / pharmacology
  • Injections, Intradermal
  • Leukotriene B4 / administration & dosage
  • Leukotriene B4 / metabolism*
  • Leukotriene B4 / pharmacology
  • Male
  • Mice
  • Mice, Inbred ICR
  • Phenylpropionates / pharmacology
  • Pruritus / metabolism*
  • Receptors, Leukotriene B4 / antagonists & inhibitors


  • Phenylpropionates
  • Receptors, Leukotriene B4
  • ONO-LB 457
  • Leukotriene B4
  • Dinoprostone