The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses

Virology. 1998 Sep 1;248(2):295-304. doi: 10.1006/viro.1998.9290.


DF-1 is a continuous cell line of chicken embryo fibroblasts. The cells are free of endogenous sequences related to avian sarcoma and leukosis viruses and have normal fibroblastic morphology. DF-1 cells support the replication of avian retroviruses; diverse oncogenes induce foci of oncogenic transformation on monolayers of DF-1, and avian leukosis viruses of envelope subgroups B, D, and C induce cell death and form plaques. The new cell line will greatly facilitate studies on oncogenic transformation and cell killing by avian viruses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Avian Leukosis Virus / physiology*
  • Cell Death*
  • Cell Line / physiology*
  • Cell Line / virology
  • Cell Transformation, Neoplastic*
  • Cells, Cultured
  • Chick Embryo
  • Chickens
  • Fibroblasts / physiology*
  • Fibroblasts / virology
  • Genes, jun / physiology
  • Oncogenes / physiology*
  • Virus Replication