Modulation of Bcl-2 expression: a potential component of estrogen protection in NT2 neurons

Neuroreport. 1998 Aug 3;9(11):2565-8. doi: 10.1097/00001756-199808030-00025.


Neuroprotective effects of estrogen have been demonstrated against a variety of cytotoxic insults. We present data here addressing a possible mechanism of estrogen neuroprotection in the human teratocarcinoma cell line NT2 terminally differentiated to a neuronal phenotype. Cell death induced by H2O2 or glutamate results in a dose-dependent cell death of NT2 neurons, while 24 h of estrogen pretreatment significantly enhances neuronal viability. Bcl-2 expression has been shown to reduce oxidative stress and prevent cell death. In NT2 neurons, Bcl-2 levels are dramatically elevated upon differentiation and are further enhanced with estrogen treatment. These results suggest that neuroprotective effects of estrogen may be related to increases in Bcl-2 expression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Western
  • Cell Death / drug effects
  • Cell Death / physiology
  • Cell Differentiation / physiology
  • Estrogens / physiology*
  • Excitatory Amino Acids / toxicity
  • Gene Expression Regulation / genetics*
  • Gene Expression Regulation / physiology*
  • Genes, bcl-2 / genetics*
  • Glutamic Acid / toxicity
  • Humans
  • Hydrogen Peroxide / toxicity
  • Neurons / physiology*
  • Oxidants / toxicity
  • Oxidative Stress
  • Tumor Cells, Cultured


  • Estrogens
  • Excitatory Amino Acids
  • Oxidants
  • Glutamic Acid
  • Hydrogen Peroxide