The effect of a new type of antibacterial temporary filling material was evaluated. Ag-Zn-Zeolite (Bactekiller, Kanebo, Japan) and SiO2 filler were incorporated into urethane acrylate monomer paste in amounts of 5/55, 10/50, 20/40 and 30/30 wt%, respectively. The present study was designed to use a dye penetration test to measure direct inhibition of bacterial growth of four oral bacteria (Streptococcus mutans, Streptococcus mitis, Streptococcus salivarius, Streptococcus sanguis). The amounts of silver and zinc ions released from these materials were measured by atomic absorption spectrophotometry. The results indicated that the occurrence of marginal leakage was low in all of these materials. These materials exhibited prominent in-vitro antibacterial activity against S. mutans and S. mitis. The Ag-Zn-Zeolite in these materials was able to release very small but detectable amounts of Ag and Zn even 4 weeks after the immersion started. The larger the amounts of Ag-Zn-Zeolite that were incorporated, the greater the release of silver and zinc. However, it appears that increasing antibacterial activity is not promoted by the higher ratio of Ag-Zn-Zeolite.