Differential expansion of the N-formylpeptide receptor gene cluster in human and mouse

Genomics. 1998 Jul 15;51(2):270-6. doi: 10.1006/geno.1998.5376.


The human formylpeptide receptor (FPR) gene cluster has three members: FPR1 and FPRL1, which are expressed in neutrophils and monocytes and encode seven-transmembrane-domain chemotactic receptors specific for N-formylpeptides, and FPRL2, whose function is unknown. The FPRL1 receptor is also a lipoxin A4 receptor. Using probes for the three human genes we have cloned six distinct mouse genes, designated Fpr1 and Fpr-rs1 through Fpr-rs5, which form a cluster on chromosome 17 in a region of conserved synteny with human chromosome 19. Fpr1 encodes a functional receptor and is clearly the orthologue of FPR1. Both Fpr-rs1 and Fpr-rs2 have higher sequence homology to FPRL1 than to FPRL2; Fpr-rs1 is 97% identical in amino acid sequence to a previously reported cDNA that encodes a lipoxin A4 receptor, whereas the putative ligand for Fpr-rs2 is unknown. Fpr-rs3, Fpr-rs4, and Fpr-rs5 appear to lack human counterparts and are most similar in sequence to FPRL1. RNA for Fpr1, Fpr-rs1, and Fpr-rs2 is present in leukocytes, spleen, and lung, whereas RNA for Fpr-rs3 was detected only in skeletal muscle. We did not detect Fpr-rs4 or Fpr-rs5 RNA in any tissue tested. Moreover, Fpr-rs5 has a stop codon in the protein-coding region corresponding to transmembrane domain VI and may not encode a functional receptor. These results suggest that the FPR gene cluster has undergone differential expansion in mammals with FPRL2, Fpr-rs2, Fpr-rs3, Fpr-rs4, and Fpr-rs5 arising after divergence of human and mouse.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping*
  • Cloning, Molecular
  • Humans
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Multigene Family / genetics*
  • Open Reading Frames / genetics
  • Organ Specificity
  • RNA, Messenger / analysis
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / genetics*
  • Receptors, Lipoxin*
  • Receptors, Peptide / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid


  • FPR2 protein, human
  • RNA, Messenger
  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • Receptors, Lipoxin
  • Receptors, Peptide

Associated data

  • GENBANK/AF071179
  • GENBANK/AF071180
  • GENBANK/AF071181
  • GENBANK/AF071182
  • GENBANK/AF071183