Abstract
Activation of peritoneal B-1 cells triggers autoimmune anemia in anti-erythrocyte Ig transgenic mice (HL mice). Numbers of peritoneal B-1 cells and Ig-producing cells were negligible in the T cell-deficient HL mice generated by the cross with RAG-2-/- mice (RAG-2-/- x HL mice). Proliferation and activation of B-1 cells in RAG-2-/- x HL mice were recovered by fetal thymus transfer, indicating involvement of T cells in B-1 cell-mediated autoimmune hemolytic anemia. Involvement of T cells in proliferation and activation of B-1 cells could be by-passed by administration of lipopolysaccharide (LPS), IL-5 or IL-10 to RAG-2-/- x HL mice. Administration of LPS elevated the serum IL-10 level in HL, RAG-2-/- x HL and normal mice. Proliferation and activation of B-1 cells were blocked by an anti-IL-10 antibody in conventionally bred as well as LPS-treated HL mice. Taken together, IL-10 plays a pivotal role in activation of peritoneal B-1 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anemia, Hemolytic, Autoimmune / immunology*
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Animals
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Antibodies, Monoclonal / pharmacology
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Autoantibodies / blood
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B-Lymphocytes / immunology*
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CD5 Antigens / immunology
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Cell Count
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Clonal Deletion / immunology
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / immunology
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Erythrocytes / immunology*
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Flow Cytometry
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Hematocrit
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Interleukin-10 / blood
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Interleukin-10 / immunology*
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Interleukin-10 / pharmacology
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Interleukin-5 / blood
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Interleukin-5 / pharmacology
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Intestinal Mucosa / immunology
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Lipopolysaccharides / pharmacology
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Lymphocyte Activation / immunology*
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Peritoneum / immunology
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T-Lymphocytes / immunology*
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Thymus Gland / embryology
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Thymus Gland / immunology
Substances
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Antibodies, Monoclonal
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Autoantibodies
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CD5 Antigens
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DNA-Binding Proteins
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Interleukin-5
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Lipopolysaccharides
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Rag2 protein, mouse
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V(D)J recombination activating protein 2
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Interleukin-10