Genetic analysis of the Caenorhabditis elegans MAP kinase gene mpk-1

Genetics. 1998 Sep;150(1):103-17.

Abstract

The Caenorhabditis elegans mpk-1 gene encodes a MAP kinase protein that plays an important role in Ras-mediated induction of vulval cell fates. We show that mutations that eliminate mpk-1 activity result in a highly penetrant, vulvaless phenotype. A double mutant containing a gain-of-function mpk-1 mutation and a gain-of-function mek mutation (MEK phosphorylates and activates MPK-1) exhibits a multivulva phenotype. These results suggest that mpk-1 may transduce most or all of the anchor cell signal. Epistasis analysis suggests that mpk-1 acts downstream of mek-2 (encodes a MEK homolog) and upstream of lin-1 (encodes an Ets transcription factor) in the anchor cell signaling pathway. Finally, mpk-1 may act together with let-60 ras in multiple developmental processes, as mpk-1 mutants exhibit nearly the same range of developmental phenotypes as let-60 ras mutants.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / enzymology*
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans Proteins*
  • Cell Differentiation
  • DNA Primers
  • Mitogen-Activated Protein Kinase 1
  • Phenotype
  • Phosphorylation
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Serine-Threonine Kinases / metabolism
  • Signal Transduction
  • Transcription Factors / genetics
  • ras Proteins / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • DNA Primers
  • Transcription Factors
  • let-60 protein, C elegans
  • Protein-Serine-Threonine Kinases
  • Mitogen-Activated Protein Kinase 1
  • mpk-1 protein, C elegans
  • ras Proteins