Discrimination of Bordetella parapertussis and Bordetella pertussis organisms from clinical isolates by PCR using biotin-labelled oligonucleotide probes

Mol Cell Probes. 1998 Aug;12(4):213-7. doi: 10.1006/mcpr.1998.0173.

Abstract

A recently developed shared-primer polymerase chain reaction (PCR) was investigated, in an ongoing pertussis surveillance study for discrimination of Bordetella parapertussis and Bordetella pertussis organisms, by using specific biotin-labelled oligonucleotide probes. From a total of 132 samples, 83 were positive by the B. parapertussis specific probe, 33 were positive by the B. pertussis specific probe and 16 samples containing Hemophilus influenzae as a negative control were below threshold by both probes. The shared-primer PCR in combination with specific oligonucleotide probes provides a rapid, sensitive and specific molecular diagnostic tool for future surveillance studies. In addition, it may be used to further investigate whether B parapertussis antigens should be added to acellular pertussis vaccines to protect against B. parapertussis infections.

MeSH terms

  • Biotin*
  • Bordetella / genetics*
  • Bordetella / isolation & purification*
  • Bordetella Infections / microbiology
  • Bordetella pertussis / genetics*
  • Bordetella pertussis / isolation & purification*
  • Humans
  • Oligonucleotide Probes*
  • Polymerase Chain Reaction / methods*
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Whooping Cough / microbiology

Substances

  • Oligonucleotide Probes
  • Biotin