Structure of the histone acetyltransferase Hat1: a paradigm for the GCN5-related N-acetyltransferase superfamily

Cell. 1998 Aug 21;94(4):427-38. doi: 10.1016/s0092-8674(00)81584-6.


We have solved the crystal structure of the yeast histone acetyltransferase Hat1-acetyl coenzyme A (AcCoA) complex at 2.3 A resolution. Hat1 has an elongated, curved structure, and the AcCoA molecule is bound in a cleft on the concave surface of the protein, marking the active site of the enzyme. A channel of variable width and depth that runs across the protein is probably the binding site for the histone substrate. A model for histone H4 binding by Hat1 is discussed in terms of possible sources of specific lysine recognition by the enzyme. The structure of Hat1 provides a model for the structures of the catalytic domains of a protein superfamily that includes other histone acetyltransferases such as Gcn5 and CBP.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyl Coenzyme A / chemistry*
  • Acetyltransferases / chemistry*
  • Acetyltransferases / genetics
  • Acetyltransferases / metabolism
  • Amino Acid Sequence
  • Arylamine N-Acetyltransferase / chemistry
  • Binding Sites
  • Catalysis
  • Crystallography
  • DNA-Binding Proteins*
  • Fungal Proteins / chemistry
  • Histone Acetyltransferases
  • Histones / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • Multigene Family
  • Protein Conformation
  • Protein Kinases / chemistry
  • Protein Structure, Secondary
  • Recombinant Proteins / chemistry
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Synchrotrons


  • DNA-Binding Proteins
  • Fungal Proteins
  • Histones
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • Acetyl Coenzyme A
  • Acetyltransferases
  • GCN5 protein, S cerevisiae
  • Histone Acetyltransferases
  • histone acetyltransferase type B complex
  • Arylamine N-Acetyltransferase
  • Protein Kinases

Associated data

  • PDB/1BOB