Detection of trisomy 3 in primary gastric B-cell lymphoma by using chromosome in situ hybridization on paraffin sections

Am J Clin Pathol. 1998 Sep;110(3):347-53. doi: 10.1093/ajcp/110.3.347.


Recent studies in Western populations have shown that trisomy 3 is the most frequent chromosomal abnormality in primary gastric lymphoma (PGL). To study the incidence of trisomy 3 and its implications for the pathogenesis of PGL in Hong Kong, we have applied the technique of chromosome in situ hybridization in 13 cases of PGL by using archival paraffin-embedded tissue sections. Five cases of chronic gastritis were used as controls. Trisomy 3 was found in 9 (69%) of 13 cases, including cases of low-grade lymphoma and high-grade lymphoma with or without a low-grade component. Our findings are similar to the incidence of trisomy 3 reported in the Western literature. The persistent finding of trisomy 3 in various histologic grades of PGL suggests that it may be useful as a clonal marker in this group of neoplasms. Various molecular events involving chromosome 3 may be related to the pathogenesis of this group of lymphomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosomes, Human, Pair 16 / genetics*
  • Chromosomes, Human, Pair 3 / genetics*
  • Gastritis / genetics
  • Gastritis / pathology
  • Hong Kong
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization / methods
  • Lymphoma, B-Cell / genetics*
  • Lymphoma, B-Cell / pathology
  • Retrospective Studies
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Trisomy*