UNC-115, a conserved protein with predicted LIM and actin-binding domains, mediates axon guidance in C. elegans

Neuron. 1998 Aug;21(2):385-92. doi: 10.1016/s0896-6273(00)80547-4.

Abstract

Axon guidance receptors modulate the growth cone cytoskeleton through signaling pathways that are not well understood. Here, we describe the C. elegans unc-115 gene, which encodes a candidate cytoskeletal linker protein that acts in axon guidance. unc-115 mutants have defects in a subset of axons, particularly as the affected axons change environments during outgrowth. The unc-115 gene encodes a putative actin-binding protein that is similar to the human actin-binding protein abLIM/limatin; it has a villin headpiece domain and three LIM domains that could mediate protein interactions. unc-115 is expressed in neurons during their development and is required cell-autonomously in certain neurons for normal axon guidance. We propose that UNC-115 modulates the growth cone actin cytoskeleton in response to signals received by growth cone receptors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / chemistry
  • Caenorhabditis elegans / physiology*
  • Conserved Sequence*
  • Culture Techniques
  • Cytoskeletal Proteins / chemistry
  • Cytoskeletal Proteins / physiology*
  • Epidermis / metabolism
  • Genetic Code
  • Helminth Proteins / chemistry
  • Helminth Proteins / physiology*
  • Humans
  • LIM Domain Proteins
  • Microfilament Proteins / biosynthesis
  • Microfilament Proteins / physiology*
  • Molecular Sequence Data
  • Morphogenesis
  • Mutation
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / physiology*
  • Neurons / metabolism
  • Protein Structure, Tertiary

Substances

  • ABLIM1 protein, human
  • Cytoskeletal Proteins
  • Helminth Proteins
  • LIM Domain Proteins
  • Microfilament Proteins
  • Nerve Tissue Proteins

Associated data

  • GENBANK/AF068867