Brain fos-like immunoreactivity in chronic decerebrate and neurologically intact rats given 2,5-anhydro-D-mannitol

Brain Res. 1998 Aug 10;801(1-2):107-15. doi: 10.1016/s0006-8993(98)00566-6.


Injection of the fructose analogue, 2,5-anhydro-d-mannitol (2,5-AM), increases food intake and Fos-like immunoreactivity (Fos-li) in both brainstem and forebrain structures. Because of the interconnections between brainstem and forebrain areas, it has not been possible to determine whether or to what extent induction of Fos-li in a given region reflects brainstem-forebrain interactions. We addressed this issue using chronic decerebrate (CD) rats with complete transections of the neuroaxis at the meso-diencephalic juncture. CD and neurologically intact control rats were injected (i.p.) with saline or 400 mg/kg 2,5-AM and brains were examined for Fos-li. Both intact and CD rats showed increased Fos-li in the nucleus of the solitary tract (NTS) after injection of 2,5-AM as compared with saline. 2, 5-AM treatment increased Fos-li in the external lateral division of parabrachial nucleus (PBNel) in intact but not in CD rats, suggesting that descending projections from the forebrain may play a role in the activation of PBNel neurons after 2,5-AM injection. Decerebration eliminated significant 2,5-AM-induced Fos-li responses in forebrain structures, including the paraventricular nucleus, supraoptic nucleus, bed nucleus of the stria terminalis and central nucleus of the amygdala. The results are consistent with the hypothesis that the activation of forebrain structures after 2,5-AM treatment is due to stimulation by ascending projections from the brainstem.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies / metabolism
  • Brain / cytology
  • Brain / drug effects*
  • Brain / physiology
  • Brain Stem / cytology
  • Brain Stem / drug effects
  • Brain Stem / physiology
  • Cell Count / drug effects
  • Decerebrate State / metabolism*
  • Eating / drug effects
  • Immunohistochemistry
  • Male
  • Mannitol / analogs & derivatives*
  • Mannitol / pharmacology
  • Neural Pathways / drug effects
  • Neural Pathways / physiology
  • Paraventricular Hypothalamic Nucleus / chemistry
  • Paraventricular Hypothalamic Nucleus / cytology
  • Paraventricular Hypothalamic Nucleus / drug effects
  • Prosencephalon / chemistry
  • Prosencephalon / cytology
  • Prosencephalon / physiology
  • Proto-Oncogene Proteins c-fos / analysis*
  • Rats
  • Rats, Sprague-Dawley
  • Solitary Nucleus / chemistry
  • Solitary Nucleus / cytology
  • Solitary Nucleus / drug effects
  • Time Factors


  • Antibodies
  • Proto-Oncogene Proteins c-fos
  • Mannitol
  • 2,5-anhydromannitol