The T-box transcription factor Brachyury regulates expression of eFGF through binding to a non-palindromic response element

Development. 1998 Oct;125(19):3887-94.

Abstract

Brachyury is a member of the T-box gene family and is required for formation of posterior mesoderm and notochord during vertebrate development. The ability of Brachyury to activate transcription is essential for its biological function, but nothing is known about its target genes. Here we demonstrate that Xenopus Brachyury directly regulates expression of eFGF by binding to an element positioned approximately 1 kb upstream of the eFGF transcription start site. This site comprises half of the palindromic sequence previously identified by binding site selection and is also present in the promoters of the human and mouse homologues of eFGF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • DNA Primers / genetics
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Female
  • Fetal Proteins*
  • Fibroblast Growth Factors / genetics*
  • Gene Expression Regulation, Developmental
  • Humans
  • Mesoderm / metabolism
  • Mice
  • Notochord / metabolism
  • Promoter Regions, Genetic
  • T-Box Domain Proteins*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Xenopus / embryology*
  • Xenopus / genetics*
  • Xenopus / metabolism

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • Fetal Proteins
  • T-Box Domain Proteins
  • Transcription Factors
  • Fibroblast Growth Factors
  • Brachyury protein