Diurnal variation in serum remnant-like lipoproteins, platelet aggregation and fibrinolysis in healthy volunteers

Haemostasis. Nov-Dec 1997;27(6):305-14. doi: 10.1159/000217471.


Postprandial triglyceridemia and 'remnantemia' may better reflect the atherosclerotic risk than triglyceride (TG) levels in the fasting state. Recently, a new method was developed based on a monoclonal antibody recognizing an epitope distal to the carboxyl end of apo B48 which allows easy measurement of remnant-like lipoproteins (RLP). This study was performed in order to investigate RLP response to a standardized fat meal and establish a normal diurnal pattern of RLP in blood and compare it to platelet aggregation and fibrinolysis in healthy young men. We investigated 7 male volunteers (age range 18-23 years) who received a standardized fat meal (Othsuka Pharmaceutical Company, Japan) containing 32.9% lipids, 2.5% protein, 2.5% carbohydrate, 0.3% calcium and 0.1% phospholipids, and 74 mg/100 g cholesterol (C) at 7:30. The energetic value of this cocktail was 341 kcal/100 g. Area under curve (AUC) responses in TG, RLP-TG and RLP-C after the meal were as follows: for TG 28.66 +/- 8.94; for RLP-TG 17.54 +/- 5.55; for RLP-C 1.27 +/- 0.42 mg x dl-1 x h-1. These responses were correlated to each other. Surprisingly, collagen-induced platelet aggregation in whole blood was negatively related to RLP-C AUC. Fluctuation patterns of TG, RLP-TG and RLP-C concentrations during the day were remarkably similar, peaking in this particular group of subjects at 10:00-12:00 and at about 23:00, whereas cholesterol was decreasing late in the night and very early in the morning. This pattern was different from those of platelet aggregation and fibrinolysis parameters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adrenocorticotropic Hormone / blood
  • Adult
  • Apolipoproteins / blood*
  • Area Under Curve
  • Cholesterol / blood
  • Circadian Rhythm / physiology*
  • Dietary Fats / administration & dosage
  • Fibrinolysis / physiology*
  • Humans
  • Hydrocortisone / blood
  • Japan
  • Lipoproteins / blood*
  • Male
  • Plasminogen Activator Inhibitor 1 / blood
  • Platelet Aggregation / physiology*
  • Time Factors
  • Tissue Plasminogen Activator / blood
  • Triglycerides / blood*


  • Apolipoproteins
  • Dietary Fats
  • Lipoproteins
  • Plasminogen Activator Inhibitor 1
  • Triglycerides
  • remnant-like particle cholesterol
  • Adrenocorticotropic Hormone
  • Cholesterol
  • Tissue Plasminogen Activator
  • Hydrocortisone