Abstract
The Xenopus origin recognition complex is essential for chromosomal DNA replication in cell-free extracts. We have immunopurified the Xenopus origin recognition complex with anti-Xorc2 antibodies and analyzed its composition and properties. Xorc2 (p63) is specifically associated with Xorc1 (p115) and up to four additional polypeptides (p81, p78, p45, and p40). The cDNA encoding p81 is highly homologous to various expressed sequence tags from humans and mice encoding a protein of previously unknown function. Immunodepletion of p81 from Xenopus egg extracts, which also results in the removal of Xorc2, completely abolishes chromosomal DNA replication. Thus, p81 appears to play a crucial role at S phase in higher eukaryotes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chromosomes / genetics
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Cloning, Molecular
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DNA Replication*
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DNA, Complementary
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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Female
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Humans
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Mice
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Molecular Sequence Data
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Molecular Weight
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Oocytes / physiology*
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Oocytes / ultrastructure
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Origin Recognition Complex
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Peptide Fragments / chemistry
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Phosphates / metabolism
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Polymerase Chain Reaction
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Sequence Tagged Sites
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Xenopus
Substances
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DNA, Complementary
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DNA-Binding Proteins
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Origin Recognition Complex
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Peptide Fragments
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Phosphates
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Recombinant Proteins