Establishment and characterization of EBV-positive and EBV-negative primary effusion lymphoma cell lines harbouring human herpesvirus type-8

Br J Haematol. 1998 Sep;102(4):1081-9. doi: 10.1046/j.1365-2141.1998.00877.x.


In this study we report on the establishment and characterization of two novel lymphoma cell lines (CRO-AP/3 and CRO-AP/5) which carry infection by human herpesvirus type-8 (HHV-8) and have derived from AIDS-related primary effusion lymphoma (PEL). These two cell lines are representative of different virologic subtypes of PEL, i.e. HHV-8+/EBV- PEL in the case of CRO-AP/3 and HHV-8+/EBV+ PEL in the case of CRO-AP/5. Consistent with the diagnosis of PEL, both CRO-AP/3 and CRO-AP/5 expressed indeterminate (i.e. non-B, non-T) phenotypes although immunogenotypic studies documented their B-cell origin. Both cell lines are devoid of genetic lesions of c-MYC, BCL-2 and p53 as well as gross rearrangements of BCL-6. Detailed histogenetic characterization of these novel PEL cell lines suggests that PEL may derive from a post-germinal centre B cell which has undergone pre-terminal differentiation. The CRO-AP/3 and CRO-AP/5 cell lines may provide a valuable model for clarifying the pathogenesis of PEL. In particular, these cell lines may help understand the relative contribution of HHV-8 and EBV to PEL growth and development and may facilitate the identification of recurrent cytogenetic abnormalities highlighting putative novel cancer related loci relevant to PEL.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Adhesion Molecules / metabolism
  • DNA-Binding Proteins / genetics
  • Embryonal Carcinoma Stem Cells
  • Herpesviridae Infections / complications
  • Herpesvirus 4, Human / isolation & purification*
  • Herpesvirus 8, Human / isolation & purification*
  • Humans
  • Immunophenotyping
  • Karyotyping
  • Lymphoma, AIDS-Related / immunology
  • Lymphoma, AIDS-Related / pathology
  • Lymphoma, AIDS-Related / virology*
  • Male
  • Mutation
  • Neoplastic Stem Cells / pathology
  • Plasma Cells / metabolism
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-bcl-6
  • Transcription Factors / genetics
  • Tumor Cells, Cultured
  • Tumor Virus Infections / complications


  • Cell Adhesion Molecules
  • DNA-Binding Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-6
  • Transcription Factors