The H-cadherin (CDH13) gene is inactivated in human lung cancer

Hum Genet. 1998 Jul;103(1):96-101. doi: 10.1007/s004390050790.


We have previously reported frequent allelic loss in chromosome bands 16q24.1-q24.2 in human lung cancer. Since the H-cadherin (CDH13) gene has been isolated and mapped to this common region of allelic loss, we have investigated this gene in human lung cancer. The reverse transcription/polymerase chain reaction technique has revealed the loss of expression in four (57%) of seven lung cancer cell lines. To study the CDH13 gene further, we have analyzed deletions, genetic alterations, and methylation status at the 5' region of this gene. Three (75%) of four cell lines that have lost expression show a deletion of the CDH13 locus accompanied by hypermethylation of the remaining allele. Moreover, hypermethylation has been observed in nine (45%) of 20 primary lung cancers. These results suggest that a combination of deletion and hypermethylation causes inactivation of the CDH13 gene in a considerable number of human lung cancers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cadherins / genetics*
  • Chromosome Deletion*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16*
  • Cloning, Molecular
  • Cosmids
  • DNA Methylation
  • Exons
  • Gene Expression Regulation, Neoplastic*
  • Genes, Tumor Suppressor*
  • Humans
  • Lung Neoplasms / genetics*
  • Molecular Sequence Data
  • Promoter Regions, Genetic


  • Cadherins
  • H-cadherin

Associated data

  • GENBANK/AB001090
  • GENBANK/AB001091
  • GENBANK/AB001092
  • GENBANK/AB001093
  • GENBANK/AB001094
  • GENBANK/AB001095
  • GENBANK/AB001096
  • GENBANK/AB001097
  • GENBANK/AB001098
  • GENBANK/AB001099
  • GENBANK/AB001100
  • GENBANK/AB001101
  • GENBANK/AB001102
  • GENBANK/AB001103