MHC-dependent and -independent activation of human nickel-specific CD8+ cytotoxic T cells from allergic donors

J Invest Dermatol. 1998 Sep;111(3):360-6. doi: 10.1046/j.1523-1747.1998.00306.x.


T lymphocytes are critical effectors in the pathogenesis of contact hypersensitivity. Nickel is the most common contact sensitizer in humans and nickel-specific CD4+ T helper cells have been extensively characterized. Because recent observations have suggested the activation of CD8+ T cells in murine models of contact hypersensitivity, we investigated the existence of CD8+ hapten-specific T lymphocytes in patients with allergy to nickel. Nickel-specific T cell lines were generated from the peripheral blood of three allergic donors. The T cell lines were composed of a majority of CD4+ T cells, but CD8+ T cells were also present and their percentage increased with repeated in vitro stimulations. In addition to nickel-reactive helper T cell-0-type or helper T cell-2-type CD4+ T cell clones, CD8+ T cell clones could be derived from these cell lines and a total of 15 clones were further studied. Cytokine production was evaluated for 11 CD8+ T cell clones that were either cytotoxic T cell-0- or cytotoxic T cell-1-type clones. Additional effector functions were investigated on the complete panel of T cell clones. These CD8+ T cells did not only display hapten-specific proliferation, but also specific cytotoxic activities towards autologous EBV-B cells in the presence of nickel. Two different types of CD8+ T cells were characterized. Most of the clones lysed only autologous targets in the constant presence of nickel; however, one clone was able to lyse numerous targets in the presence of NiSO4, irrespective of the expression of either major histocompatibility complex class I or class II molecules. The characterization of nickel-specific cytotoxic CD8+ T cells with different requirements for nickel-specific target lysis, may have important implications in the development or in the control of human contact hypersensitivity reactions to nickel in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Donors*
  • CD4 Lymphocyte Count
  • Cells, Cultured
  • Clone Cells
  • Dermatitis, Contact / immunology*
  • Histocompatibility Antigens Class I / blood
  • Histocompatibility Antigens Class II / blood
  • Humans
  • Lymphocyte Activation*
  • Lymphocyte Count
  • Major Histocompatibility Complex / immunology*
  • Nickel / pharmacology*
  • T-Lymphocytes, Cytotoxic / immunology*


  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Nickel