Centromeric protein B null mice are viable with no apparent abnormalities

Dev Biol. 1998 Sep 15;201(2):135-43. doi: 10.1006/dbio.1998.9005.


The centromere protein B (CENP-B) is a centromeric DNA/binding protein. It recognizes a 17-bp sequence motif called the CENP-B box, which is found in the centromeric region of most chromosomes. It binds DNA through its amino terminus and dimerizes through its carboxy terminus. CENP-B protein has been proposed to perform a vital role in organizing chromatin structures at centromeres. However, other evidence does not agree with this view. For example, CENP-B is found at inactive centromeres on stable dicentric chromosomes, and also mitotically stable chromosomes lacking alpha-satellite DNA have been reported. To address the biological function of CENP-B, we generated mouse null mutants of CENP-B by homologous recombination. Mice lacking CENP-B were viable and fertile, indicating that mice without CENP-B undergo normal somatic and germline development. Thus, both mitosis and meiosis are able to proceed normally in the absence of CENP-B.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoantigens*
  • Cell Line
  • Centromere Protein B
  • Chromosomal Proteins, Non-Histone / chemistry
  • Chromosomal Proteins, Non-Histone / physiology*
  • Crosses, Genetic
  • DNA-Binding Proteins / physiology*
  • Fertility / genetics
  • Fetal Viability / genetics
  • Genotype
  • Histocytochemistry
  • Immunohistochemistry
  • Litter Size
  • Male
  • Mice
  • Mice, Knockout
  • Microtubules / metabolism
  • RNA, Messenger / metabolism
  • Sperm Count
  • Testis / cytology


  • Autoantigens
  • Cenpb protein, mouse
  • Centromere Protein B
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • RNA, Messenger