In vitro and in vivo prolonged biological activities of novel vitamin C derivative, 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G), in cosmetic fields

J Nutr Sci Vitaminol (Tokyo). 1998 Jun;44(3):345-59. doi: 10.3177/jnsv.44.345.


The biological activity of the novel vitamin C derivative, 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G), was evaluated in vitro and in vivo. The percutaneous absorption of AA-2G was determined in five Japanese males. The excretion of ascorbic acid (AA) in the subjects administered AA-2G was sustained for a longer period than in the subjects administered ascorbic acid 2-phosphate (AA-2P), which is a conventional vitamin C derivative. An analysis of the distribution of AA in the skin showed that small black specks assumed to be AA were observed in the epidermis even 3 d after applying AA-2G. The melanin synthesis in B16 melanoma cells was inhibited more by AA-2G than by AA-2P, and AA-2G also prevented more UV-induced damage of human skin keratinocytes and fibroblasts than AA-2P did. From these in vivo and in vitro results, it is supposed that the conversion of AA-2G to AA is sustained for a long time compared with that of AA-2P, and that AA-2G is an effective and available compound having vitamin C activity in human subjects.

MeSH terms

  • Absorption
  • Ascorbic Acid / analogs & derivatives*
  • Ascorbic Acid / analysis
  • Ascorbic Acid / metabolism
  • Ascorbic Acid / pharmacokinetics
  • Ascorbic Acid / pharmacology
  • Ascorbic Acid / therapeutic use
  • Cells, Cultured
  • Cosmetics*
  • Epidermis / metabolism
  • Humans
  • Hydroxyl Radical / metabolism
  • Keratinocytes / drug effects
  • Keratinocytes / radiation effects
  • Lipid Peroxidation / drug effects
  • Male
  • Melanins / biosynthesis
  • Melanoma / metabolism
  • Skin / chemistry
  • Skin / metabolism
  • Sunburn / prevention & control
  • Tissue Distribution
  • Tumor Cells, Cultured
  • Ultraviolet Rays


  • Cosmetics
  • Melanins
  • ascorbic acid 2-O-glucoside
  • Hydroxyl Radical
  • Ascorbic Acid