Isolation of human intestinal defensins from ileal neobladder urine

FEBS Lett. 1998 Sep 4;434(3):272-6. doi: 10.1016/s0014-5793(98)00994-6.


We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Blood Proteins / chemistry
  • Blood Proteins / urine*
  • Defensins
  • Humans
  • Ileum / metabolism*
  • Ileum / transplantation
  • Molecular Sequence Data
  • Paneth Cells / metabolism
  • Phospholipases A / metabolism
  • Protein Processing, Post-Translational
  • Urinary Reservoirs, Continent*


  • Blood Proteins
  • Defensins
  • Phospholipases A