Production of parathyroid hormone-related peptide by synovial fibroblasts in human osteoarthritis

FEBS Lett. 1998 Aug 21;433(3):331-4. doi: 10.1016/s0014-5793(98)00940-5.


Synovial fibroblasts from patients with osteoarthritis in culture produced parathyroid hormone-related peptide (PTHrP) on treatment with phorbol ester (TPA) in a dose- and time-dependent manner. The levels of PTHrP immunoreactivity in the conditioned medium of synovial fibroblast cultures were measured using specific PTHrP antibody. The maximum production was obtained at a concentration of 10(-8) M and 24 h after TPA treatment. But sensitivity to TPA of synovial fibroblasts differed among four patients from slight to marked. PTHrP production was also induced with inflammatory cytokines, such as 1 ng/ml of IL-1alpha, IL-1beta, IL-6 and TNF-alpha, and 10(-6) M prostaglandin E2, after 24 h treatment. The expression of PTHrP was confirmed by reverse-transcriptase polymerase chain reaction. Since the synovial fibroblasts isolated from osteoarthritic patients produce high levels of IL-6 and IL-8, typical cytokines produced in synovial fibroblasts, production of PTHrP may provide new insight into the pathophysiology of joint disorder.

MeSH terms

  • Cells, Cultured
  • Cytokines / pharmacology*
  • Cytokines / physiology
  • Dinoprostone / pharmacology
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Gene Expression Regulation / drug effects
  • Humans
  • Interleukin-1 / pharmacology
  • Interleukin-6 / pharmacology
  • Kinetics
  • Osteoarthritis / metabolism*
  • Parathyroid Hormone-Related Protein
  • Polymerase Chain Reaction
  • Protein Biosynthesis
  • Proteins / genetics*
  • Recombinant Proteins / pharmacology
  • Synovial Membrane / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Time Factors
  • Tumor Necrosis Factor-alpha / pharmacology


  • Cytokines
  • Interleukin-1
  • Interleukin-6
  • PTHLH protein, human
  • Parathyroid Hormone-Related Protein
  • Proteins
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Dinoprostone
  • Tetradecanoylphorbol Acetate