Dendritic cells from skin and blood of macaques both promote SIV replication with T cells from different anatomical sites

J Med Primatol. Apr-Jun 1998;27(2-3):121-8. doi: 10.1111/j.1600-0684.1998.tb00236.x.

Abstract

The SIV-macaque system offers the opportunity to study the pathogenesis and immune aspects of a primate retroviral infection in which immunodeficiency also develops, much like HIV infection in humans. Since it is known that human dendritic cells (DCs) are involved in HIV replication, mature cytokine-generated DCs obtained from precursors in the blood and skin-derived DCs were isolated from healthy rhesus macaques and compared with respect to their ability to support SIV infection. Here, it is shown for both skin- and blood-derived DCs that i) virus production depends on both DCs and T cells, ii) this occurs similarly with T cells from blood, skin, spleen, or lymph nodes, and iii) DCs can transmit virus equally to syngeneic and allogeneic T cells. No differences between DCs from skin or blood were observed. Therefore, the easily accessible blood-derived DCs of macaques provide an appropriate population to study the role of DCs in immunodeficiency virus infection.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Cells, Cultured
  • Coculture Techniques
  • Dendritic Cells / cytology
  • Dendritic Cells / drug effects
  • Dendritic Cells / virology*
  • Female
  • Gene Products, gag / biosynthesis
  • Giant Cells
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Humans
  • Interleukin-4 / pharmacology
  • Lymph Nodes / immunology
  • Macaca mulatta
  • Recombinant Proteins / pharmacology
  • Simian Immunodeficiency Virus / physiology*
  • Skin / cytology*
  • Spleen / immunology
  • T-Lymphocytes / cytology
  • T-Lymphocytes / virology*
  • Virus Replication*

Substances

  • Gag protein p27, Simian immunodeficiency virus
  • Gene Products, gag
  • Recombinant Proteins
  • Interleukin-4
  • Granulocyte-Macrophage Colony-Stimulating Factor