Intracellular cytokine expression in the CD4+ and CD8+ T cells from intestinal mucosa of simian immunodeficiency virus infected macaques

J Med Primatol. Apr-Jun 1998;27(2-3):129-40. doi: 10.1111/j.1600-0684.1998.tb00237.x.

Abstract

Isolated intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) from jejunum of SIV infected animals were examined for alterations in basal cytokine expression by RT-PCR. Remarkable changes in IFNgamma and IL-10 RNA levels were observed in IEL and LPL in SIV infection while IL-4 and IL-2 RNA levels remained unaltered. In addition, the CD4+ and CD8+ LPL were examined for intracellular cytokine production following mitogenic activation by flowcytometry. Both CD4+ and CD8+ T lymphocytes in intestinal mucosa retained the potential to produce IFNgamma in response to mitogenic stimulation in vitro, without a remarkable change in IL-4 production. The dominant IFNgamma cytokine response could be one of the major contributing factors in SIV associated enteropathy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Cytokines / biosynthesis
  • Cytokines / genetics*
  • Flow Cytometry
  • Gene Expression Regulation
  • In Situ Hybridization
  • Intestinal Mucosa / immunology*
  • Intestinal Mucosa / virology
  • Jejunum / immunology
  • Jejunum / virology
  • Macaca mulatta
  • Reverse Transcriptase Polymerase Chain Reaction
  • Simian Acquired Immunodeficiency Syndrome / immunology*
  • Simian Immunodeficiency Virus / isolation & purification*

Substances

  • Cytokines