Characterization of bovine and human cDNAs encoding NAP-22 (22 kDa neuronal tissue-enriched acidic protein) homologs

Mol Cells. 1998 Aug 31;8(4):471-7.


We have characterized the bovine and the human cDNAs encoding the NAP-22 (22 kDa neuronal tissue-enriched acidic protein) homologs. Both bovine and human cDNAs encode proteins of 227 amino acids. The deduced amino acid sequences of the bovine and the human proteins are 63% and 65% identical, respectively, to that of rat NAP-22 protein, strongly suggesting that both the cDNAs characterized encode NAP-22 proteins. They also share 45% and 41% amino acid sequence identities with chicken CAP-23 (23 kDa cytoskeleton associated protein). Several important protein motifs, including myristoylation and phosphorylation sites, are well conserved in sequences and positions in all three mammalian NAP-22 proteins and chicken CAP-23 proteins. The bovine cDNA was characterized further. Southern analysis of the bovine genomic DNA suggests that the bovine NAP-22 protein is encoded by a single-copy gene. RNA blot analysis revealed that the bovine gene for NAP-22 protein encodes a 1.7 kb transcript that is present only in the brain. Our data suggest that the four proteins, bovine and human NAP-22 homologs, rat NAP-22, and chicken CAP-23, have homologous functions in different organisms.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism
  • Calmodulin-Binding Proteins / genetics*
  • Cattle
  • DNA / analysis
  • DNA / genetics
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics*
  • Dinucleotide Repeats
  • Female
  • Gene Dosage
  • Genes / genetics
  • Humans
  • Liver / metabolism
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Ovary / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Retina / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • 3' Untranslated Regions
  • Calmodulin-Binding Proteins
  • DNA, Complementary
  • Nerve Tissue Proteins
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/AF039656
  • GENBANK/U92535