The monoclonal antibody AS02 recognizes a protein on human fibroblasts being highly homologous to Thy-1

Arch Dermatol Res. 1998 Jul;290(7):360-6. doi: 10.1007/s004030050318.


Recently, we described a novel fibroblast-restricted monoclonal antibody (mAb AS02) that recognizes a membrane-bound antigen. Characterization and isolation of the corresponding antigen showed that mAb AS02 recognized a protein on human fibroblasts that is highly homologous or identical to human Thy-1 antigen (CD90). Partial amino acid sequencing of the corresponding mAb AS02 antigen and comparison with known proteins revealed a 100% homology of the sequenced peptides to the human Thy-1 antigen. Cross-immunodepletion studies with mAb AS02 and an anti-Thy-1 antibody confirmed these results. Utilizing two-dimensional (2D) gel electrophoresis of fibroblast cell extracts and purified antigen, mAb AS02 and the anti-Thy-1-antibody recognized identical protein spots. Furthermore, we demonstrated many identical biochemical properties of the corresponding AS02 antigen and Thy-1 antigen, such as the molecular weight of the core protein and deglycosylation products and the detection of a GPI anchor. In functional assays, the attachment of fibroblasts to collagen I and fibronectin was increased after incubation of fibroblasts with mAb AS02. Therefore, the Thy-1 antigen appears to be involved in the regulation of the adherence of human dermal fibroblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / immunology*
  • Blotting, Western
  • Cell Adhesion / immunology
  • Cell-Free System / immunology
  • Fibroblasts / cytology
  • Fibroblasts / immunology*
  • Humans
  • Membrane Proteins / chemistry
  • Membrane Proteins / immunology*
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Thy-1 Antigens / immunology*


  • Antibodies, Monoclonal
  • Membrane Proteins
  • Thy-1 Antigens