Expression of adhesion molecules in cultured human nasal mucosal microvascular endothelial cells activated by interleukin-1 beta or tumor necrosis factor-alpha: effects of dexamethasone

Int Arch Allergy Immunol. 1998 Sep;117(1):68-77. doi: 10.1159/000023992.

Abstract

Adhesion molecules of microvascular endothelial cells play a key role in the inflammatory processes involved in nonallergic sinusitis. We investigated the cytokine-regulated expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1), and the effect of dexamethasone on these expressions in cultured human nasal microvascular endothelial cells (HNMEC). ICAM-1 was enhanced, and E-selectin and VCAM-1 were induced in a dose-dependent fashion following stimulation with IL-1beta or TNF-alpha. HNMEC differed from human umbilical vein endothelila cells in that (1) maximal upregulation of ICAM-1 expression induced by IL-1beta or TNF-alpha required more time (2) TNF-alpha was more potent than IL-1beta in VCAM-1 expression, and (3) dexamethasone inhibited the upregulation of E-selectin expression alone. These findings contribute to a better understanding of the characteristic features of leukocyte infiltration into inflamed tissue and the effect of glucocorticoid in nonallergic chronic sinusitis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion Molecules / biosynthesis*
  • Cells, Cultured
  • Dexamethasone / pharmacology*
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects*
  • Endothelium, Vascular / metabolism
  • HL-60 Cells / drug effects
  • Humans
  • Interleukin-1 / pharmacology*
  • Interleukin-1 / physiology
  • Microcirculation / drug effects
  • Nasal Mucosa / blood supply*
  • Nasal Mucosa / cytology
  • Nasal Mucosa / drug effects*
  • Prospective Studies
  • Random Allocation
  • Tumor Necrosis Factor-alpha / pharmacology*
  • Tumor Necrosis Factor-alpha / physiology
  • Umbilical Veins

Substances

  • Cell Adhesion Molecules
  • Interleukin-1
  • Tumor Necrosis Factor-alpha
  • Dexamethasone