PRAME, a gene encoding an antigen recognized on a human melanoma by cytolytic T cells, is expressed in acute leukaemia cells

Br J Haematol. 1998 Sep;102(5):1376-9. doi: 10.1046/j.1365-2141.1998.00982.x.


Gene PRAME was found to encode an antigen recognized on a human melanoma cell line by an autologous cytolytic T-lymphocyte clone. This gene is expressed at a high level in a very large fraction of tumours, such as melanomas, non-small-cell lung carcinomas, sarcomas, head and neck tumours and renal carcinomas. It is therefore a candidate for tumour immunotherapy even though some low expression is found in certain normal tissues. We tested by RT-PCR the expression of PRAME on more than 250 bone marrow or blood samples from patients with a haematological malignancy. Approximately 25% of the acute leukaemia samples were positive. Remarkably, all acute myeloblastic leukaemias that carried the chromosomal translocation t(8;21), which fuses the genes AML1 and ETO, expressed PRAME at a high level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Antigens, Neoplasm / genetics
  • Antigens, Neoplasm / metabolism*
  • Chromosome Aberrations
  • Gene Expression
  • Humans
  • Leukemia / metabolism*
  • Melanoma / metabolism*
  • RNA, Neoplasm / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • T-Lymphocytes / metabolism*


  • Antigens, Neoplasm
  • RNA, Neoplasm