Three-dimensional structure of an evolutionarily conserved N-terminal domain of syntaxin 1A

Cell. 1998 Sep 18;94(6):841-9. doi: 10.1016/s0092-8674(00)81742-0.


Syntaxin 1A plays a central role in neurotransmitter release through multiple protein-protein interactions. We have used NMR spectroscopy to identify an autonomously folded N-terminal domain in syntaxin 1A and to elucidate its three-dimensional structure. This 120-residue N-terminal domain is conserved in plasma membrane syntaxins but not in other syntaxins, indicating a specific role in exocytosis. The domain contains three long alpha helices that form an up-and-down bundle with a left-handed twist. A striking residue conservation is observed throughout a long groove that is likely to provide a specific surface for protein-protein interactions. A highly acidic region binds to the C2A domain of synaptotagmin I in a Ca2+-dependent interaction that may serve as an electrostatic switch in neurotransmitter release.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Surface / chemistry*
  • Antigens, Surface / genetics*
  • Antigens, Surface / metabolism
  • Calcium / metabolism
  • Calcium-Binding Proteins / metabolism
  • Evolution, Molecular*
  • Magnetic Resonance Spectroscopy
  • Membrane Glycoproteins / metabolism
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry*
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Neurotransmitter Agents / metabolism
  • Protein Binding / physiology
  • Protein Folding
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Rats
  • Sequence Homology, Amino Acid
  • Synaptic Transmission / physiology
  • Synaptotagmin I
  • Synaptotagmins
  • Syntaxin 1


  • Antigens, Surface
  • Calcium-Binding Proteins
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • Neurotransmitter Agents
  • Stx1a protein, rat
  • Synaptotagmin I
  • Syntaxin 1
  • Syt1 protein, rat
  • Synaptotagmins
  • Calcium

Associated data

  • PDB/1BR0