Use of random amplified polymorphic DNA (RAPD) for generating specific DNA probes for oxyuroid species (Nematoda)

Parasite. 1998 Mar;5(1):47-50. doi: 10.1051/parasite/1998051047.

Abstract

Random amplified DNA markers (RAPD; Williams et al., 1990) were used to obtained specific RAPD fragments characterising different species of oxyuroids. We tested six species of worms parasitizing vertebrates or invertebrates: Passalurus ambiguus Rudolphi, 1819, parasite of Leporids; Syphacia obvelata (Rudolphi, 1802) Seurat, 1916, a parasite of rodents; Blatticola blattae (Graeffe, 1860) Chitwood, 1932 parasite of the cockroach Blattella germanica; Hammerschmidtiella diesingi (Hammerschmidt, 1838) Chitwood, 1932 and Thelastoma bulhoesi (Magalhaes, 1990) Travassos, 1929, parasites of the cockroach Periplaneta americana, and an undescribed parasite species of a passalid insect from New Caledonia. Among 15 oligonucleotides tested, nine produced several specific bands allowing the interspecific discrimination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cockroaches / parasitology
  • DNA Primers / chemistry
  • DNA Probes / chemistry*
  • DNA Probes / genetics
  • DNA, Helminth / analysis*
  • DNA, Helminth / genetics
  • Female
  • Insecta / parasitology
  • Lagomorpha / parasitology
  • Male
  • Muridae / parasitology
  • Oxyuroidea / classification
  • Oxyuroidea / genetics*
  • Polymerase Chain Reaction
  • Random Amplified Polymorphic DNA Technique*
  • Reproducibility of Results
  • Species Specificity

Substances

  • DNA Primers
  • DNA Probes
  • DNA, Helminth