Spectrophotometric and electrochemical determinations of L(+)-lactate in blood by use of lactate dehydrogenase from yeast

Clin Chem. 1976 Nov;22(11):1802-5.

Abstract

Lactate can be determined rapidly in blood by spectrophotometric and amperometric (enzyme electrode) procedures based on its oxidation by ferricyanide, the reaction being catalyzed with yeast L(+)-lactate dehydrogenase (cytochrome b2) (EC 1.1.2.3). In the photometric method lactate can be measured in a few minutes, but blood samples must first be deproteinized. In the amperometric procedure no treatment of blood is needed except ferricyanide addition. The enzyme electrode we used has a response time shorter than 1 min when its critical variables are optimized. Preliminary standardization is reduced to minimum operation, because electrode response is proportional to lactate concentration over a wide range (0.1 to 8.0 mol/liter) and many determinations can be done with little cost in enzyme. A simple electrical device ("two-electrode device") is described that is well suited for furture micro-cell construction. Lactate determinations on a series of normal blood samples show no deviation between results by these new methods and the usual ultraviolet spectrophotometric lactate tests.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Electrochemistry
  • Humans
  • Kinetics
  • L-Lactate Dehydrogenase* / metabolism
  • Lactates / blood*
  • Methods
  • Muscles / enzymology
  • Pichia / enzymology
  • Spectrophotometry, Ultraviolet / methods
  • Stereoisomerism

Substances

  • Lactates
  • L-Lactate Dehydrogenase