Glucocorticoids decrease cytochrome c oxidase activity of isolated rat kidney mitochondria

FEBS Lett. 1998 Sep 11;435(1):25-8. doi: 10.1016/s0014-5793(98)01033-3.

Abstract

The importance of mitochondria is rising as a target in pathologic processes such as ischemia. We have investigated the effects of hydrocortisone, prednisolone, dexamethasone and triamcinolone on oxidative phosphorylation, Ca2+ fluxes, swelling and membrane potentials in isolated kidney mitochondria. The measurement of respiration state 3 showed a significant decrease in presence of glucocorticoids whereas the other respiration states were not modified. When mitochondria were uncoupled and either the complexes III and IV or the complex IV were stimulated, the O2 consumption was decreased by glucocorticoids. These results suggest the cytochrome c oxidase is a target of the glucocorticoid effect on the respiratory chain. Indeed, the other mitochondrial functions investigated were unchanged, ruling out a direct effect on Ca2+ fluxes or swelling. A regulation of cytochrome c oxidase activity by glucocorticoids will be of particular interest in pathology involving metabolic insult.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • Calcium / metabolism
  • Dexamethasone / pharmacology
  • Electron Transport Complex IV / drug effects*
  • Electron Transport Complex IV / metabolism*
  • Enzyme Activation / drug effects
  • Glucocorticoids / pharmacology*
  • Hydrocortisone / pharmacology
  • Kidney / drug effects
  • Kidney / enzymology*
  • Kidney / physiology
  • Male
  • Membrane Potentials / drug effects
  • Mitochondria / enzymology*
  • Mitochondria / physiology
  • Mitochondrial Swelling / drug effects
  • Oxygen Consumption / drug effects
  • Prednisolone / pharmacology
  • Rats
  • Rats, Wistar
  • Triamcinolone / pharmacology

Substances

  • Glucocorticoids
  • Triamcinolone
  • Dexamethasone
  • Prednisolone
  • Electron Transport Complex IV
  • Calcium
  • Hydrocortisone