Tumour-specific MHC-class-II-restricted responses after in vitro sensitization to synthetic peptides corresponding to gp100 and Annexin II eluted from melanoma cells

Cancer Immunol Immunother. 1998 Sep;47(1):32-8. doi: 10.1007/s002620050501.


In a search for potentially tumour-specific MHC-class-II-restricted antigens, the immunogenicity of endogenous peptides that had been eluted from HLA-DR molecules of the human melanoma cell line FM3 (HLA-DRB1*02x, DRB1*0401) was tested in vitro. Two 16-mers representing gp100 positions 44-59, and annexin II positions 208-223 bound well to isolated DRB1*0401 molecules and are discussed here. HLA-DR-matched normal donors' T cells were cultured with peptide-pulsed artificial antigen-presenting cells (CHO cells cotransfected with genes for HLA-DRB1*0401 and CD80 and coexpressing high levels of both human molecules). Specific sensitization was achieved against both peptides, as measured in assays of autocrine proliferation and interleukin-2 secretion. Moreover, responses to native autologous melanoma cells but not to autologous B cells were also observed. In view of the expression of fas by the activated T cells and of fas ligand by the melanoma cells, blockade of potential fas/ fas-ligand interactions was undertaken using monoclonal antibodies (mAb). The antagonistic fas-specific mAb M3, but not the fas agonist M33, caused a markedly enhanced T cell response to FM3 cells. These results demonstrate that synthetic peptide antigens are able to sensitize T cells in vitro for effective MHC-class-II-restricted recognition of melanoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Annexin A2 / immunology*
  • Antibodies, Neoplasm / immunology*
  • Antibody Specificity
  • CHO Cells / metabolism
  • Cricetinae
  • Histocompatibility Antigens Class II / pharmacology*
  • Humans
  • Immunization
  • Melanoma, Experimental / immunology
  • Melanoma, Experimental / pathology
  • Membrane Glycoproteins / immunology*
  • Neoplasm Proteins / immunology*
  • Peptide Fragments / biosynthesis
  • Tumor Cells, Cultured
  • gp100 Melanoma Antigen


  • Annexin A2
  • Antibodies, Neoplasm
  • Histocompatibility Antigens Class II
  • Membrane Glycoproteins
  • Neoplasm Proteins
  • PMEL protein, human
  • Peptide Fragments
  • gp100 Melanoma Antigen