The Tat protein of equine infectious anemia virus (EIAV) activates cellular gene expression by read-through transcription

Gene. 1998 Sep 28;219(1-2):25-35. doi: 10.1016/s0378-1119(98)00389-8.


The Tat protein of equine infectious anemia virus, EIAV, was shown to augment viral gene expression, presumably through interaction with the Tat responsive element, TAR. Recently, cell-free polyadenylation assays suggested that perturbation of the EIAV TAR secondary structure diminished polyadenylation efficiency. The present study indicates that the EIAV TAR regulates the efficiency of the 3'-end processing of viral RNA also in transfected cells. Moreover, our data suggest that the provision of the EIAV Tat protein in trans potentiates read-through transcription through the 3' viral long terminal repeat (3' LTR), thus suggesting activation of downstream-located cellular genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell-Free System
  • DNA Primers
  • Dogs
  • Gene Expression Regulation*
  • Gene Products, tat / metabolism*
  • Genes, tat
  • Horses
  • Mutagenesis, Site-Directed
  • Open Reading Frames
  • Polymerase Chain Reaction
  • Protein Structure, Secondary
  • RNA Precursors / genetics
  • RNA Processing, Post-Transcriptional
  • RNA, Viral / genetics*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Repetitive Sequences, Nucleic Acid
  • Transcription, Genetic*
  • Transfection


  • DNA Primers
  • Gene Products, tat
  • RNA Precursors
  • RNA, Viral
  • Recombinant Proteins