Whole sequence of spoIIIE-like, sporulation-inhibitory, and transfer gene (spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus and detection of spi-like gene in the actinomycete chromosome

Biosci Biotechnol Biochem. 1998 Aug;62(8):1597-600. doi: 10.1271/bbb.62.1597.

Abstract

The nucleotide sequence of spoIIIE-like and the sporulation-inhibitory and transfer gene (spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus were examined to detect the promoter region. Using Southern blotting and a spi fragment as probe, spi-like genes were detected in chromosomes of the host and other actinomycetes. These results suggest that there is a spi- and spoIIIE-like gene in chromosomes of some actinomycetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / chemistry
  • Actinomycetales / genetics*
  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Base Sequence
  • Blotting, Southern
  • Chromosomes, Bacterial / ultrastructure
  • Conjugation, Genetic / genetics*
  • Molecular Sequence Data
  • Plasmids / chemistry
  • Promoter Regions, Genetic / genetics
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sigma Factor*
  • Spores, Bacterial / chemistry
  • Streptomyces / chemistry*
  • Streptomyces / genetics
  • Streptomyces / physiology
  • Transcription Factors*

Substances

  • Bacterial Proteins
  • Sigma Factor
  • Transcription Factors
  • spi protein, actinomycetes
  • spoIIR protein, Bacillus subtilis
  • spore-specific proteins, Bacillus