Blood-filled spaces with and without filler materials in guided bone regeneration. A comparative experimental study in the rabbit using bioresorbable membranes

Clin Oral Implants Res. 1997 Apr;8(2):75-81. doi: 10.1034/j.1600-0501.1997.080201.x.


The aim of the present study was to evaluate the effect of natural deproteinized bone mineral on the temporal and spatial pattern of bone formation in a guided bone regeneration model system while using a bioresorbable membrane device. A periosteal skin flap was raised uncovering the calvaria of 20 rabbits. A stiff hemispherical dome made of polylactic acid was placed onto the roughened calvaria and anchored by screws. Prior to placement, the dome was either filled with peripheral blood (control group, 8 rabbits) or with blood and OsteoGraf/N-300 (test group, 12 rabbits). At 1 month, histologic sections revealed bone regeneration in both test and control domes to various degrees. In the test domes, bone height reached 78% (67-83) and bone volume was 11% (6-17), while in the control domes, bone height was 45% (14-67) and bone volume 6% (1-11). At 2 months, bone height was unchanged in the test group at 70% (67-83) and bone volume had only slightly increased to 16% (11-21). In the controls, height increased to 86% (60-100) and volume to 20% (9-27). Thus, in this model system, natural bone mineral fill contributed to accelerate initial bone neogenesis, while it did not contribute to increasing bone volume or bone height at later observation stages.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biodegradation, Environmental
  • Blood
  • Bone Regeneration*
  • Bone Substitutes*
  • Bone Transplantation / methods
  • Cattle
  • Guided Tissue Regeneration / methods*
  • Lactic Acid
  • Membranes, Artificial*
  • Polyesters
  • Polymers
  • Rabbits
  • Skull
  • Statistics, Nonparametric


  • Bone Substitutes
  • Membranes, Artificial
  • Polyesters
  • Polymers
  • Lactic Acid
  • poly(lactide)