Abstract
One enzyme which catalyzes the last step of the formation of the hormone retinoic acid from vitamin A (retinol) is retinal dehydrogenase type II (Ra1DH2). Ra1DH2, expressed in the Escherichia coli BL21(DE3) strain, was purified and crystallized using ammonium sulfate as a precipitant. These crystals belong to the space group P212121 (a = 108, b = 150, c = 168 A, alpha = beta = gamma = 90 degrees).
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aldehyde Oxidoreductases / chemistry*
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Aldehyde Oxidoreductases / isolation & purification
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Animals
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Crystallization
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Crystallography, X-Ray
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Isoenzymes / chemistry*
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Isoenzymes / isolation & purification
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / isolation & purification
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Retinal Dehydrogenase
Substances
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Isoenzymes
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Recombinant Fusion Proteins
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Aldehyde Oxidoreductases
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Retinal Dehydrogenase