Purification, crystallization and preliminary X-ray diffraction studies of retinal dehydrogenase type II

Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):639-42. doi: 10.1107/s0907444997014121.

Abstract

One enzyme which catalyzes the last step of the formation of the hormone retinoic acid from vitamin A (retinol) is retinal dehydrogenase type II (Ra1DH2). Ra1DH2, expressed in the Escherichia coli BL21(DE3) strain, was purified and crystallized using ammonium sulfate as a precipitant. These crystals belong to the space group P212121 (a = 108, b = 150, c = 168 A, alpha = beta = gamma = 90 degrees).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aldehyde Oxidoreductases / chemistry*
  • Aldehyde Oxidoreductases / isolation & purification
  • Animals
  • Crystallization
  • Crystallography, X-Ray
  • Isoenzymes / chemistry*
  • Isoenzymes / isolation & purification
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / isolation & purification
  • Retinal Dehydrogenase

Substances

  • Isoenzymes
  • Recombinant Fusion Proteins
  • Aldehyde Oxidoreductases
  • Retinal Dehydrogenase