Localisation of a novel adhesion blocking epitope on the human beta 1 integrin chain

Cell Adhes Commun. 1998 Jun;5(4):257-71. doi: 10.3109/15419069809040296.


Members of the beta 1 integrin family mediate cellular adherence to a wide range of extracellular and cell surface associated ligands. Conformational changes have been shown to be associated with integrin activation and ligand binding. Some studies suggest that there may be a restricted region of the beta 1 integrin that serves as the target for regulatory antibodies which can inhibit or stimulate integrin function. Here we identify an inhibitory epitope that is located at a distinct sight from that suggested for other inhibitory antibodies. Three different adhesion blocking antibodies, JB1A, C30B, and D11B bind to a peptide corresponding to residues 82-87 of the mature beta 1 chain. Mn++ inhibited the binding of JB1A to purified beta 1 integrin. In contrast the binding of several other antibodies to beta 1 were not influenced by these conditions. JB1A binding to purified peptide was also inhibited by Mn++ suggesting that it related to interference with the antibody function rather than a cation dependent change in the epitope. Our data 1) directly demonstrates the peptide sequence recognised by three adhesion blocking antibodies to the human beta 1 integrin chain 2) identifies a novel epitope located at residues 82-87, distinct from that of previously described regulatory epitopes 3) characterises a Mn++ sensitive antibody integrin interaction. Collectively, these results indicate the existence of multiple regulatory sites on the beta 1 integrin molecule.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / pharmacology
  • Antibody Specificity*
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology
  • Cloning, Molecular
  • Epitope Mapping*
  • Gene Library
  • Humans
  • Integrin beta1 / analysis*
  • Integrin beta1 / genetics*
  • Integrin beta1 / immunology
  • Jurkat Cells / chemistry
  • Jurkat Cells / cytology
  • Jurkat Cells / physiology
  • Magnesium / pharmacology
  • Molecular Sequence Data
  • Peptide Fragments / pharmacology
  • Recombinant Fusion Proteins / immunology


  • Antibodies, Monoclonal
  • Integrin beta1
  • Peptide Fragments
  • Recombinant Fusion Proteins
  • Magnesium