Growth factor protection against cytokine-induced apoptosis in neonatal rat islets of Langerhans: role of Fas

FEBS Lett. 1998 Sep 18;435(2-3):207-10. doi: 10.1016/s0014-5793(98)01051-5.


Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or insulin-like growth factor-1 (10(-8)M) gave only partial protection against cell killing, but prevented the Fas-mediated component. In the absence of cytokine treatment, Fas-mediated killing was not observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Apoptosis / immunology*
  • Cells, Cultured
  • Hypoglycemic Agents / pharmacology*
  • Insulin / pharmacology*
  • Insulin-Like Growth Factor I / pharmacology*
  • Interferon-gamma / pharmacology
  • Islets of Langerhans / immunology
  • Islets of Langerhans / pathology*
  • Rats
  • Tumor Necrosis Factor-alpha / pharmacology
  • fas Receptor / immunology*


  • Hypoglycemic Agents
  • Insulin
  • Tumor Necrosis Factor-alpha
  • fas Receptor
  • Insulin-Like Growth Factor I
  • Interferon-gamma