Cytotoxicity induced by recombinant human tumor necrosis factor-alpha dependent on the types of its receptors on canine cells

J Vet Med Sci. 1998 Aug;60(8):889-95. doi: 10.1292/jvms.60.889.


Based on the recent findings that show how recombinant human tumor necrosis factor (rh-TNF)-alpha has potent antitumor activity on human cancer patients when it locally administrated, we have tested the cytotoxicity of rh-TNF-alpha on 3 canine cultured cells: (1) canine kidney carcinoma (CKCa-1), (2) mastocytoma and (3) Mardin Darby canine kidney cells (MDCK). The cell surface expression of TNF-alpha receptors on these canine cells was also determined with anti-human TNF RI and RII polyclonal antibodies. Our data shows that on CKCa-1 which has TNF RI receptors rh-TNF-alpha induced cytotoxicity. By contrast, it exhibited no toxicity on canine mastocytoma which has mainly RII receptors. The data also suggest actinomycin D (ACT-D), an anticancer antibiotic, enhanced the cytotoxicity of rh-TNF-alpha. Combined with ACT-D, rh-TNF-alpha showed the cytotoxicity on MDCK which possessed both TNF RI and RII receptors. The results indicate that the cytotoxicity of rh-TNF-alpha depends on the presence of TNF RI receptors on canine tumor cells.

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival / drug effects*
  • Dog Diseases / pathology
  • Dogs
  • HeLa Cells
  • Humans
  • Kidney
  • Kidney Neoplasms / pathology
  • Kidney Neoplasms / veterinary
  • Lymphocytes / cytology
  • Lymphocytes / drug effects
  • Mast-Cell Sarcoma / pathology
  • Mast-Cell Sarcoma / veterinary
  • Recombinant Proteins / toxicity*
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / physiology
  • Tumor Necrosis Factor-alpha / toxicity*


  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha