Short consensus repeat domain 1 of decay-accelerating factor is required for enterovirus 70 binding

J Virol. 1998 Nov;72(11):9380-3. doi: 10.1128/JVI.72.11.9380-9383.1998.

Abstract

Enterovirus 70 (EV70), like several other human enteroviruses, can utilize decay-accelerating factor (DAF [CD55]) as an attachment protein. Using chimeric molecules composed of different combinations of the short consensus repeat domains (SCRs) of DAF and membrane cofactor protein (CD46), we show that sequences in SCR1 of DAF are essential for EV70 binding. Of the human enteroviruses that can bind to DAF, only EV70 and coxsackievirus A21 require sequences in SCR1 for this interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD / chemistry
  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • Binding Sites / genetics
  • CD55 Antigens / chemistry
  • CD55 Antigens / genetics
  • CD55 Antigens / metabolism*
  • Consensus Sequence
  • Enterovirus / metabolism*
  • HeLa Cells
  • Humans
  • Membrane Cofactor Protein
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Mice
  • Receptors, Virus / chemistry
  • Receptors, Virus / genetics
  • Receptors, Virus / metabolism
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Repetitive Sequences, Amino Acid

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • CD46 protein, human
  • CD55 Antigens
  • Mcp protein, mouse
  • Membrane Cofactor Protein
  • Membrane Glycoproteins
  • Receptors, Virus
  • Recombinant Fusion Proteins