cDNA cloning and expression of a novel serine protease, TLSP

Biochim Biophys Acta. 1998 Aug 20;1399(2-3):225-8. doi: 10.1016/s0167-4781(98)00116-x.

Abstract

A cDNA for a putative novel serine protease, TLSP, was cloned from human hippocampus cDNA with polymerase chain reaction based strategies. The putative amino acid sequence of TLSP is similar to the trypsin-type serine proteases. TLSP mRNA is expressed in keratinocytes. Overexpressed TLSP protein in neuro2a cells was detected in culture medium.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / biosynthesis*
  • DNA, Complementary / chemistry
  • Hippocampus / enzymology*
  • Humans
  • Keratinocytes / enzymology*
  • Molecular Sequence Data
  • Plasmids
  • Sequence Alignment
  • Serine Endopeptidases / biosynthesis
  • Serine Endopeptidases / genetics*
  • Trypsin / biosynthesis
  • Trypsin / chemistry
  • Trypsin / genetics*

Substances

  • DNA, Complementary
  • trypsin-like serine protease
  • Serine Endopeptidases
  • Trypsin

Associated data

  • GENBANK/AB012917