Epstein-Barr virus LMP2A drives B cell development and survival in the absence of normal B cell receptor signals

Immunity. 1998 Sep;9(3):405-11. doi: 10.1016/s1074-7613(00)80623-8.


Epstein-Barr virus (EBV) establishes a persistent latent infection in peripheral B lymphocytes in humans and is associated with a variety of malignancies and proliferative disorders. Latent membrane protein 2A (LMP2A) is one of only two viral proteins expressed in latently infected B lymphocytes in vivo. LMP2A blocks B cell receptor (BCR) signal transduction in vitro by binding the Syk and Lyn protein tyrosine kinases. To analyze the significance of LMP2A expression in vivo, transgenic mice with B cell lineage expression of LMP2A were generated. LMP2A expression results in the bypass of normal B lymphocyte developmental checkpoints allowing immunoglobulin-negative cells to colonize peripheral lymphoid organs, indicating that LMP2A possesses a constitutive signaling activity in nontransformed cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / virology*
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / drug effects
  • Bone Marrow Cells / immunology
  • Cell Differentiation
  • Cell Survival
  • Cells, Cultured
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain / drug effects
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain / physiology
  • Genes, RAG-1 / physiology
  • Herpesvirus 4, Human / chemistry*
  • Herpesvirus 4, Human / immunology*
  • Interleukin-7 / pharmacology
  • Lymphocyte Activation / drug effects
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Receptors, Antigen, B-Cell / physiology*
  • Signal Transduction
  • Transgenes / physiology
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / physiology*


  • EBV-associated membrane antigen, Epstein-Barr virus
  • Interleukin-7
  • Receptors, Antigen, B-Cell
  • Viral Matrix Proteins